属性
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熔点
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116-126°C
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敏感性
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对湿度和热敏感
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溶解性
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Soluble in water, and methanol
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存贮条件
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储存温度-20°C；充氩保存
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密度
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1.3700
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描述
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应用
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IPTG 常用于需要诱导 β-半乳糖苷酶活性的克隆实验。它常与 X-Gal 或 Bluo-Gal 结合使用，用于重组细菌菌落的蓝白筛选，这些菌落可以诱导 lac 操纵子在大肠杆菌中的表达。IPTG 与 lacI 阻遏蛋白结合并改变其构象而发挥作用，防止 β-半乳糖苷酶编码基因 lacZ 的抑制。
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产品介绍
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IPTG, Dioxane-Free (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli. The compound is widely used with X-Gal plates to screen for blue and white colonies. The lac operon system and IPTG have been used in multiple cloning experiments and cellular systems to study gene activity.
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备注
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非代谢性半乳糖类似物。
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别名
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异丙基-β-D-硫代半乳糖吡喃糖苷; 异丙基-β-D-硫代半乳糖吡喃糖甙; 异丙基1-硫代-β-D-半乳糖吡喃糖苷; 异丙基-β-D-硫代半乳糖苷; 异丙基 β-D-1-硫代半乳糖吡喃糖苷; IPTG;IPTG; Isopropyl 1-Thio-β-D-galactopyranoside; Isopropyl-β-D-thiogalactopyranoside
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