|
Novex™ TBE Gels 8% provide high-resolution analysis of restriction digests and PCR products. Designed to run on the XCell SureLock™ Mini-Cell, the polyacrylamide gels give sharp, clearly resolved, intense bands, and provide separations of double-strand DNA fragments from 25–220 bp.
Novex TBE Gels are available in a variety of well formats and gel percentages and can be stained by silver staining, ethidium bromide, and SYBR™ Green staining techniques after electrophoresis.
Advantages of TBE Gels for nucleic acid separation:
• High resolution and sensitivity with lower background staining
• Requires ∼10% sample concentration and volume of large or agarose gels
• Efficient blotting
• Easy extraction of DNA from gels
• Gel extraction does not interfere with enzymatic reactions
• Accurate and reproducible results
Formulation
Novex TBE gels are manufactured with high-purity Tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS.
Recommended buffers
For optimum performance, Novex™ TBE Running Buffer and Novex™ Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex Hi-Density TBE Sample Buffer contains the tracking dyes Bromophenol Blue and Xylene Cyanol as well as the density agent Ficoll™, which yields sharper and straighter bands than conventional density agents.
|